Composite

Part:BBa_K3799001

Designed by: Shubhamay Das   Group: iGEM21_IISER_Kolkata   (2021-09-30)


EiCsm6 under IPTG inducible promoter with double terminator

This part encodes for IPTG inducible expression of Crispr protein,csm6.It consist of coding sequence of csm6 from Enterococcus italicus(BBa_K3799000) under IPTG inducible EColi promoter Pveg(BBa_J04500) along with strong RBS(BBa_B0034) and double terminator(BBa_B0015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
    Illegal XhoI site found at 587
    Illegal XhoI site found at 1274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None